The book 'Biotechnology' is designed for graduate courses in Biotechnology, Botany, Zoology, Life Sciences and Agriculture that have a paper (either full or in part) in biotechnology. The book has 18 chapters that cover, recombinant DNA technology, animal cell culture, animal biotechnology, plant tissue culture, plant biotechnology, transgenic technology (both animals and plants), biotechnology in health care, industrial microbiology, enzyme technology, food, beverage and fuel biotechnology, environmental biotechnology, genomics and bioinformatics, biosafety, and intellectual property rights. The book has been written in simple language in a style I followed for teaching my students. The concepts have been explained with the help of suitable diagrams and every effort has been made to maintain readability and facilitate easy comprehension. It is expected to serve as the basic reading resource for the students and would be helpful in various competitive examinations.