The second edition of the experimental manual includes simulation package in the form of computer based experiments, made available to the students online. The text format of the experimental manual that provides details of each experiment and also the step-by-step approach to access the experiments, as per the order on the online platform has been expanded for each experiment in this edition. \n\nThe online access serves as an adjunct to the normal practice of noting down the observations, calculations, interpretations, etc. of different experiments, as they are taught in the amphibian practical classes. The students can use the blank pages provided on the left hand side of the manual for this purpose. Thus this manual, along with accompanying online resource can serve dual purpose.